服务项目 (Service Items)

周期（周）

Time(weeks)

免疫库

immunized library

天然库

naive library

杂交瘤制备筛选

Hybridoma Screening

多肽设计与合成

Peptide design & synthesis

多肽设计

Peptide design

~2

可选，免费

多肽合成

Peptide synthesis

√

√

小分子或多肽偶联

conjugation

√

√

蛋白制备

proteins preparation

cDNA合成

cDNA synthesis

6~8

√

另议，时间与价格均不包含在以下信息中

载体构建

Vector construction

√

√

蛋白表达与纯化

Protein expression & purification

√

√

免疫阶段

Immunization

免疫动物

Immunized animals

~8

√

√

√

√

ELISA检测

Elisa detection

√

√

√

√

细胞融合与筛选

Fusion & Screening

1.5

√

√

亚克隆阶段

subcloning

1

√

√

腹水生产（2只大鼠）

Ascites production (Injection on 2 mice)

1

√

√

cDNA制备

cDNA preparation

逆转录制备cDNA文库

prepare cDNA liberary by RT-PCR

~1

√

√

cDNA 文库质检

cDNA liberary QC check

√

√

构建Fab抗体库

Fab liberary construction

VH与VL基因获得

obtained VH and VL genes

10~15

√

√

构建初级抗体库

primary antibody liberary construction

√

√

构建Fab抗体库

Fab liberary construction

√

√

噬菌体筛选

phage screening

噬菌体颗粒包装

phage particles package

10~15

√

√

√

√

磁珠亲和筛选

magnetic beeds affinity screening

√

√

√

√

阳性克隆回收基因测序
Recover positive clones and DNA sequencing

√

√

√

√

抗体表达

Antibody expression

特异性双链基因合成与Fc基因桥接

synthetic of target phage gene and Fc gene

~5

√

√

√

√

构建真核表达载体

Eukaryotic system expression plasmids construction

√

√

√

√

真核表达

Eukaryotic system expression

√

√

√

√

稳转细胞系构建        
Stably transfected cell line construction

纯化与检测

Purification and detection

标签纯化

Lable tag affinity purification

~1

√

√

√

√

亲和纯化

Affinity purification

√

√

特异性亲和纯化

Antigen specific affinity purification

√

√

√

√

总周期（月）

Total lead time(months)

7~9