服务项目(Service Items)

周期（月）Time(months)

客户提供(Start point)

纯化的单一亚型抗体

purified single isotype antibodies

混合抗体（血清）

mixed antibodies (serum)

原核表达从头制备

Prokaryotic proteins synthesis de novo

真核表达从头制备
 Eukaryotic proteins synthesis de novo

抗原制备

antigens preparation

cDNA合成 

 cDNA synthesis

1~2

另议，时间与价格均不包含在以下信息中

载体构建

Vector construction

√

√

√

√

分离特定亚型并纯化

Isolating single isotype antibodies and purification

√

蛋白表达与纯化

Protein expression & purification

√

√

√

√

酶切回收

 Enzyme treatment and recovering

√

√

√

√

免疫阶段

Immunization

免疫5只小鼠或两只兔子       

Immunized 5 mice or 2 rabbits

1.5

√

√

√

√

√

√

√

√

ELISA效价检查                                      

ELISA titer check

√

√

√

√

√

√

√

√

细胞融合与筛选

Fusion & Screening

1.5

√

√

√

√

同家族免疫球蛋白正向复筛（亚克隆阶段）

Same isotype immunoglobal protein positive screening (subcloning)

√

√

√

√

腹水生产（3只小鼠）                    

Ascites production (Injection on 2 mice)

0.5

√

√

√

√

非同家族免疫球蛋白负筛

Different isotype immunoglobal protein negative screenibg

0.5

√

√

√

√

√

√

√

√

腹水生产（5只小鼠）

Ascites production (Injection on 3 mice)

1

√

√

√

√

纯化与检测

Purification and detection

0.5

√

√

√

√

√

√

√

√

总周期（月）                                                          Total lead time(months)

4~6

6~8

4~6

6~8

5~7

6.5~8.5

>6

>8