服务项目

(Service Items)

周期（周）

Time(weeks)

客户提供 (Start point)

多肽peptide

小分子

Molecular

磷酸基/甲基/

乙基化多肽

Phosphate/Methyl/

acetyl-specific

多肽设计与合成

Peptide design & synthesis

多肽设计

Peptide design

~2

可选，免费

多肽合成 

 Peptide synthesis

√

小分子或多肽偶联

conjugation

√

√

√

免疫阶段

Immunization

免疫2只鸡

Immunized 2 chickens

~8

√

√

√

√

√

√

ELISA检测 

Elisa detection

√

√

√

√

√

√

cDNA制备

cDNA preparation

逆转录制备cDNA文库prepare cDNA liberary by RT-PCR

~1

√

√

√

√

√

√

cDNA文库质检     cDNA liberary QC check

√

√

√

√

√

√

构建Fab抗体库

Fab liberary construction

VH与VL基因获得 obtained VHand VLgenes

10~15

√

√

√

√

√

√

构建初级抗体库 primary antibody liberary construction

√

√

√

√

√

√

构建Fab抗体库Fab liberary construction

√

√

√

√

√

√

噬菌体筛选

phage screening

噬菌体颗粒包装    phage particles package

10~15

√

√

√

√

√

磁珠亲和筛选   magnetic beeds affinity screening

√

√

√

√

√

阳性克隆回收基因测序
Recover positive clones and DNA sequencing

√

√

√

√

√

抗体表达

Antibody expression

特异性双链基因合成与Fc基因桥接

synthetic of target phage gene and Fc gene

~5

√

√

√

√

√

√

构建真核表达载体

Eukaryotic system expression plasmids construction

√

√

√

√

√

√

真核表达

Eukaryotic system expression

√

√

√

√

√

稳转细胞系构建        
Stably transfected cell line construction

√

纯化与检测

Purification and detection

标签纯化

Lable tag affinity purification

~1

√

√

√

特异性亲和纯化

Antigen specific affinity purification

√

√

√

总周期（月）                                                          Total lead time(months)

7~9