促销价:3500
品牌: ATCC细胞
货号: NK-92
生长状态: 悬浮生长
运输方式: T25瓶或干冰
器官来源: 血液系统
是否是肿瘤细胞: 是
细胞形态: 聚团生长
物种来源: 人源
组织来源: 血液
Novagen Catlog: NGT-CRL-2407
Cell name: NK-92
ATCC Number: CRL-2407
Depositors: Conkwest Inc.
Biosafety Level: 2
Growth property: Suspension, multicell aggregates
Morphology: Lymphoblast
Species: Homo sapiens (human)
Source: Cell type: natural killer cell; NK cell
Disease: malignant non-Hodgkin's lymphoma
Antigen Expressed: CD2 +, CD7 +, CD11a +, CD28 + , CD45 +, CD54 +, CD56 +, CD1 -, CD3 -,
CD4 -, CD5 -, CD8 -, CD10 -, CD14 -, CD16 -, CD19 -, CD20 -, CD23 -, CD34 -,
HLA-DR -
Ethnic origin: Caucasian
Age: 50 years
Gender: Male
Growth medium: Alpha Minimum Essential medium without ribonucleosides and deoxyribo-nucleosides
but with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate + 0.2 mM inositol + 0.1 mM 2-mercaptoethanol + 0.02 mM folic acid + 100-200 U/ml recombinant IL-2 + 12.5% horse serum + 12.5% fetal bovine serum
Temperature: 37.0℃
Subculturing: Subcultivation Ratio: 1:2 to 1:3 is recommended Subculture every 2 to 3 days
Comments: NK-92 is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral
blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma.
The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/ml is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2.
The cell line is cytotoxic to a wide range of malignant cells; it kills both K562 cells and Daudi cells in chromium release assays.
NK-92 cells (after irradiation to prevent proliferation) can be used effectively for immunological ex vivo purging of leukemia from blood without compromising hematopoietic cell function.
NK-92 cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR.
Species determination: STR analysis (intraspecies) Human (Unique DNA Profile)
TH01: 6, 9.3
D5S818: 12 13
D13S317: 9, 12
D7S820: 10, 11
D16S539: 11, 12
CSF1PO: 11, 12
Amelogenin: X, Y
vWA: 18
TPOX: 8
1 March 2016 | Volume 7 | Article 91
Review
published: 14 March 2016
doi: 10.3389/fimmu.2016.00091
Frontiers in Immunology | www.frontiersin.org
1 November 2015 | Volume 6 | Article 578
Review
published: 17 November 2015
doi: 10.3389/fimmu.2015.00578
Frontiers in Immunology | www.frontiersin.org
该细胞在各大杂志上有报道,并且美国食品药品监督管理局(FDA)和伦理委员会(IRB)已经批准使用指定细胞库提供的无病原体NK-92细胞,在德国进行的临床试验,且报道临床二期的数据较为理想。
本株NK-92细胞由我公司安诺伦生物于16年2月引进,传代清晰,已做STR基因分型鉴定报告,并为该株细胞配套专用NK92无血清培养体系,使得这株细胞培养更为容易。
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